Absolute position (with WT) Mutation Hu-R/CHO Mu-R/CHO 25 (G) A 8.53 10.12 25 (G) D 12.67 16.09 25 (G) E 6.33 8.98 25 (G) G 7.95 12.21 25 (G) H 6.01 7.63 25 (G) I 8.44 10.69 25 (G) K 7.08 10.19 25 (G) L 4.55 6.57 25 (G) N 6.44 8.08 25 (G) P 6.39 9.62 25 (G) Q 5.45 7.13 25 (G) R 11.53 13.61 25 (G) S 7.53 8.88 25 (G) T 5.24 6.65 25 (G) V 7.13 9.05 25 (G) W 7.08 8.98 25 (G) Y 7.21 9.70 26 (F) A 6.92 8.98 26 (F) D 6.62 8.71 26 (F) E 5.83 7.62 26 (F) F 7.37 9.40 26 (F) G 2.40 4.43 26 (F) H 6.38 8.79 26 (F) I 7.97 11.03 26 (F) K 4.02 6.31 26 (F) L 6.68 10.04 26 (F) N 8.43 11.16 26 (F) P 9.21 11.62 26 (F) Q 7.54 10.11 26 (F) R 2.87 5.28 26 (F) S 6.09 7.55 26 (F) T 7.69 8.44 26 (F) V 6.64 10.56 26 (F) W 5.28 6.79 26 (F) Y 5.81 8.97 27 (T) D 8.45 13.84 27 (T) E 8.90 12.12 27 (T) G 4.79 6.10 27 (T) H 8.34 13.09 27 (T) H 6.45 8.67 27 (T) I 6.05 8.34 27 (T) K 6.03 9.59 27 (T) L 6.47 9.68 27 (T) N 5.36 7.00 27 (T) N 3.23 6.09 27 (T) Q 2.66 3.53 27 (T) R 4.64 7.71 27 (T) S 7.99 13.12 27 (T) T 5.75 9.24 27 (T) V 5.40 8.98 27 (T) W 6.50 8.22 27 (T) Y 4.38 7.48 28 (F) A 7.78 11.86 28 (F) D 2.36 4.37 28 (F) E 2.66 5.72 28 (F) F 7.59 11.11 28 (F) G 8.82 10.40 28 (F) H 10.06 14.22 28 (F) I 8.30 10.77 28 (F) K 2.12 4.29 28 (F) P 5.75 7.78 28 (F) Q 5.08 7.54 28 (F) R 2.22 3.89